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Carola Tilgmann

Driftchef

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Suppression of growth defects of α-amylase secreting Escherichia coli by signal sequence fusion

Författare

  • Ilari Suominen
  • Jarmo Käpylä
  • Carola Tilgmann
  • Virpi Glumoff
  • Pekka Mäntsälä

Summary, in English

Two fusions of the Bacillus stearothermophilus α-amylase gene (amyS) with lacpoZ′ were constructed. The first, being a transcriptional fusion, placed amyS directly under lac promoter control eliminating interference by the endogenous promoter. IPTG induction of amyS transcription in this construction resulted in liberation of periplasmic proteins and eventually cell lysis. The other fusion replaced eight N-terminal amino acid residues from the signal sequence by 11 residues from the lacZ′ moiety in pUC-18. Translation initiation signals were also replaced by those from lacpoZ′. In this case IPTG induction resulted in secretion of approx. 35% of total α-amylase activity in the growth medium after 24 h growth without detectable cell lysis.

Publiceringsår

1988

Språk

Engelska

Sidor

3-7

Publikation/Tidskrift/Serie

FEMS Microbiology Letters

Volym

55

Issue

1

Dokumenttyp

Artikel i tidskrift

Förlag

Oxford University Press

Nyckelord

  • Bacillus stearothermophilus
  • Escherichia coli
  • Periplasmic protein
  • Protein secretion
  • α-amylase

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0378-1097