Webbläsaren som du använder stöds inte av denna webbplats. Alla versioner av Internet Explorer stöds inte längre, av oss eller Microsoft (läs mer här: * https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Var god och använd en modern webbläsare för att ta del av denna webbplats, som t.ex. nyaste versioner av Edge, Chrome, Firefox eller Safari osv.

Default user image.

Carola Tilgmann

Driftchef

Default user image.

Expression of enzymatically active rat liver and human placental catechol-O-methyltransferase in Escherichia coli; purification and partial characterization of the enzyme

Författare

  • Kenneth Lundström
  • Carola Tilgmann
  • Johan Peränen
  • Nisse Kalkkinen
  • Ismo Ulmanen

Summary, in English

To produce sufficient amounts of recombinant catechol-O-methyltransferase (COMT) for structural and functional studies the coding regions of the rat liver and human placental COMT genes have been introduced into a bacterial expression vector pKEX14. Recombinant COMT was produced in Escherichia coli up to 10% of total bacterial protein after the induction of the T7 RNA polymerase gene with isopropyl-β-d-thiogalactopyranoside. Both the rat and human enzymes were enzymatically active, soluble and reacted with anti-COMT antiserum in Western blotting. Both enzymes were purified from E. coli cells and partially characterized by determining their specific activity, apparent molecular weight and pI.

Publiceringsår

1992-01-06

Språk

Engelska

Sidor

149-154

Publikation/Tidskrift/Serie

Biochimica et Biophysica Acta, Gene Structure and Expression

Volym

1129

Issue

2

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Nyckelord

  • (E. coli)
  • Active recombinant protein
  • Bacterial expression
  • Catechol-O-methyltransferase
  • Protein purification

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0167-4781