Carola Tilgmann
Driftchef
Expression of enzymatically active rat liver and human placental catechol-O-methyltransferase in Escherichia coli; purification and partial characterization of the enzyme
Författare
Summary, in English
To produce sufficient amounts of recombinant catechol-O-methyltransferase (COMT) for structural and functional studies the coding regions of the rat liver and human placental COMT genes have been introduced into a bacterial expression vector pKEX14. Recombinant COMT was produced in Escherichia coli up to 10% of total bacterial protein after the induction of the T7 RNA polymerase gene with isopropyl-β-d-thiogalactopyranoside. Both the rat and human enzymes were enzymatically active, soluble and reacted with anti-COMT antiserum in Western blotting. Both enzymes were purified from E. coli cells and partially characterized by determining their specific activity, apparent molecular weight and pI.
Publiceringsår
1992-01-06
Språk
Engelska
Sidor
149-154
Publikation/Tidskrift/Serie
Biochimica et Biophysica Acta, Gene Structure and Expression
Volym
1129
Issue
2
Dokumenttyp
Artikel i tidskrift
Förlag
Elsevier
Nyckelord
- (E. coli)
- Active recombinant protein
- Bacterial expression
- Catechol-O-methyltransferase
- Protein purification
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0167-4781