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Carola Tilgmann

Driftchef

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Purification methods of mammalian catechol-O-methyltransferase

Författare

  • Carola Tilgmann
  • Ismo Ulmanen

Summary, in English

The protein purification strategies used for obtaining homogeneous rat and human soluble catechol-O-methyltransferase (S-COMT) polypeptides are reviewed. Expression and purification of recombinant rat and human S-COMT in Escherichia coli and for human S-COMT in baculovirus-infected insect cells made it possible to elucidate the S-COMT polypeptides in more detail. The application of these purification methods has allowed the crystallization of the rat S-COMT protein and the analysis of the kinetic properties of the enzyme in great detail. The availability of the pure S-COMT protein together with the structural data has also greatly enhanced the development of more potent COMT inhibitors.

Publiceringsår

1996-07-20

Språk

Engelska

Sidor

147-161

Publikation/Tidskrift/Serie

Journal of chromatography. B, Biomedical applications

Volym

684

Issue

1-2

Dokumenttyp

Artikel i tidskrift

Förlag

Elsevier

Ämne

  • Biochemistry and Molecular Biology
  • Cell Biology

Nyckelord

  • catechol-O-methyltransferase
  • enzymes
  • reviews
  • protein purification

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 1572-6495