Carola Tilgmann
Driftchef
Crystallization and preliminary X-ray investigation of a recombinant form of rat catechol O-methyltransferase
Författare
Summary, in English
Rat catechol O-methyltransferase cDNA was introduced into an E. coli expression vector pKEX14, which utilizes the inducible T7 promoter. Active and soluble recombinant catechol O-methyltransferase was produced in bacteria and purified to electrophoretic homogeneity by chromatographic procedures. The purified enzyme has been crystallized by the method of vapor diffusion using polyethylene glycol as precipitant. The space group is P3121 or P3221 with a = b = 51.3 Å and c = 168.5 Å and one molecule in the asymmetric unit. The crystals diffract beyond 3.2 Å and are suitable for three-dimensional X-ray structure determination.
Avdelning/ar
- Lunds universitet
Publiceringsår
1991
Språk
Engelska
Sidor
233-236
Publikation/Tidskrift/Serie
Proteins
Volym
11
Issue
3
Dokumenttyp
Artikel i tidskrift
Förlag
John Wiley & Sons Inc.
Ämne
- Pharmaceutical Chemistry
- Structural Biology
Nyckelord
- catechol O-methyltransferase
- protein crystals
- protein structure
- X-ray crystallography
Status
Published
ISBN/ISSN/Övrigt
- ISSN: 0887-3585