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Carola Tilgmann

Driftchef

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Crystallization and preliminary X-ray investigation of a recombinant form of rat catechol O-methyltransferase

Författare

  • J. Vidgren
  • C. Tilgmann
  • K. Lundstrom
  • A. Liljas

Summary, in English

Rat catechol O-methyltransferase cDNA was introduced into an E. coli expression vector pKEX14, which utilizes the inducible T7 promoter. Active and soluble recombinant catechol O-methyltransferase was produced in bacteria and purified to electrophoretic homogeneity by chromatographic procedures. The purified enzyme has been crystallized by the method of vapor diffusion using polyethylene glycol as precipitant. The space group is P3121 or P3221 with a = b = 51.3 Å and c = 168.5 Å and one molecule in the asymmetric unit. The crystals diffract beyond 3.2 Å and are suitable for three-dimensional X-ray structure determination.

Avdelning/ar

  • Lunds universitet

Publiceringsår

1991

Språk

Engelska

Sidor

233-236

Publikation/Tidskrift/Serie

Proteins

Volym

11

Issue

3

Dokumenttyp

Artikel i tidskrift

Förlag

John Wiley & Sons Inc.

Ämne

  • Pharmaceutical Chemistry
  • Structural Biology

Nyckelord

  • catechol O-methyltransferase
  • protein crystals
  • protein structure
  • X-ray crystallography

Status

Published

ISBN/ISSN/Övrigt

  • ISSN: 0887-3585